cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kota surakarta,
Jawa tengah
INDONESIA
Nexus Kedokteran Translasional
Published by Universitas Sebelas Maret
ISSN : -     EISSN : -     DOI : -
Core Subject : Health,
Medicine & Pharmacology
Arjuna Subject : -
Articles 15 Documents
 1   2 
Search results for , issue "Vol 6, No 1 (2017): Nexus Kedokteran Translasional" : 15 Documents clear
Effect of Tofu Fermented by Rhizopus sp. on Lowering Blood Glucose Level in White Rats (Rattus norvegicus) Melati, Eldaa Putik Bunga; Wiboworini, Budiyanti; Subariyanti, Briandani
Nexus Kedokteran Translasional Vol 6, No 1 (2017): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (21.79 KB)

Abstract

Introductions : Controlling of blood glucose would be an important prevention to prevent many complications of DM. Consuming fiber is one way to maintain blood glucose in normal level. These amount of fiber could be obtained in tofu fermented by Rhizopus sp. This research aimed to know the effect of tofu fermented by Rhizopus sp. in controlling blood glucose level of diabetic induced white rats (Rattus norvegicus). Methods : This research was a laboratoric-experimental study. The subjects were Rattus norvegicus grouped randomly. The total 30 Rattus norvegicus were injected by Alloxan 15mg/ 100 gram BW, then randomly allocated into 5 group, positive control group (glibenclamide 0,05 mg/100 gram BW/day), negative control group (aquadest), and treatment groups with different doses of fermented tofu (0,025 gram/100 gram BW/day; 0,050 gram/100 gram BW/day; 0,100 gram/100 gram BW/day). The research design was Pretest and Posttest Control Group Design. The fasting blood glucose were measured before Alloxan injection (day-2), after Alloxan injection (day-5), and after the treatments (day-19). Result : ANOVA showed that there was a significant decrease of blood glucose level in all treatment groups and positive control (p = 0,001). Post Hoc Test showed that there was significant difference between all treatment groups and positive control group compared to negative control group (p = 0,001). Conclusion : Tofu fermented by Rhizopus sp. was able to decrease blood glucose level in diabetic induced white rats (Rattus norvegicus). Keyword : Tofu fermented by Rhizopus sp, blood glucose level, white rats, Alloxan, glibenclamide
Potency of Indonesian Herbal Compounds as Human Flavin Containing Monooxygenase 3 Inhibitor for Atherosclerosis Prevention Nurfitria, Fadhila Balqis; Wulandari, RAJ Sri; Indarto, Dono
Nexus Kedokteran Translasional Vol 6, No 1 (2017): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.209 KB)

Abstract

Introduction: Atherosclerosis complication in the cardiovascular system has been one of the biggest medical problems in recent years and the definitive treatment has not been found. Trimethylamine-N-oxide (TMAO) is a catalytic product of Flavin-Containing Monooxydase (FMO) 3 enzyme and might act as a predispostion factor for atherosclerosis. Indonesia has many herbal plants which can potentially be developed into antiatherosclerosis drug. This was an initial study of drug development which aimed to identify FMO3 inhibitors from Indonesian herbal plants by using molecular docking. Methods: It was a  bioinformatics study which utilized all herbal compounds recorded in HerbalDB, had three dimentional structure, and met the criteria for Lipinskis rule of five. Methimazole was used as a standard ligand and hFMO model was determined using FMO protein template from Methylophaga aminisulfidivorans. Herbal compounds were molecularly docked with hFMO3 models using AutodockVina 1.1.2. PyMOL 1.7 dan Chimera 1.10rc were used for visualization of docking results. Binding affinity, binding site, and Lipinskis rule of five criterias were used to determine hFMO3 inhibitor candidates of herbal compounds. Results: Methimazole bound to the hFMO3 model  at Asn194 with binding energy average of -3.8 kcal/mol. Droserone, vanillic acid, (s)-(+) abscisic acid, and sebacic acid had lower binding energy, had similar binding site, and had the best drug like property, compared with methimazole. Conclusion: Droserone, vanillic acid, (s)-(+) absicic acid, and sebacic acid become the potential candidates of hFMO3 inhibitor in silico. A future study using flexible ligand and flexible receptor docking methods is needed to get more accurate results. Keywords: Atherosclerosis, Molecular Docking, FMO3 inhibitor, Indonesian Herbal Plants
The Effectiveness of the Leaf Extract of Jati Belanda (Guazuma ulmifolia Lamk) as Dengue Antiviral In Vitro Pratiwi, Yani Dwi; Saptawati, Leli; Marufah, Siti
Nexus Kedokteran Translasional Vol 6, No 1 (2017): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.209 KB)

Abstract

Introduction: Dengue Haemorrhagic Fever is one of a serious disease in the world because it can cause death. During all these years, DHF in Indonesia only treated by symptomatic and supportive therapy. The purpose of this study was to determine the effectiveness of Jati Belanda leaf’s extracts as antiviral dengue in vitro. Methods: The method used is pure experimental research and the research design method is post test only control group design. The subject is dengue virus serotype 2 strain New GuineaC (DENV2 NGC) obtained from the Laboratory of Virology and Molecular Biology Department of Microbiology, Faculty of Medicine, University of Indonesiaand this research was started from June until November 2016. The independent variables in this study is the concentration of Jati Belanda leaf’s extracts obtained from Pharmaceutical Laboratory of Faculty Pharmacy, Gadjah Mada University and dependent variable are infectivity value and viability value. The collected data are presented in tables. Results:In concentration 40 µg/ml, the infectivity value is 12.6% and the viability value is 91.4%. Conclusions: The leaf extracts of Jati Belanda is potentially effective as antiviral dengue. Keywords: dengue, Jati Belanda extract, antivirus, DHF  
A Potential Candidate of Lactate Dehydrogenase Inhibitor Derived from Indonesia Herbal Compounds Nasrullah, Adam Haviyan; Indarto, Dono; Pesik, Riza Novierta; Wulandari, R. AJ. Sri
Nexus Kedokteran Translasional Vol 6, No 1 (2017): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.209 KB)

Abstract

Introduction: Lactate dehydrogenase A (LDHA) is an enzyme that catalyzes  pyruvate into lactate. LDHA plays an important role in promotion of cancer cells growth through increasing aerobic glycolysis. Because LDHA has a central role in energy metabolism, it become a molecular target for development of anticancer drug. This was a  biocomputational study that aimed to identify Indonesian herbal compounds which became a potential candidate of LDHA inhibitor via molecular docking analysis. Methods: Samples in this study were Indonesian herbal compounds that met the following criteria: (1) Registered on Database Herbal Indonesia, (2) had three-dimensional structure, and (3) met the criteria Lipinski rule of five. Oxamate used as a ligand standard and was validated using Autodock Vina software. Herbal compounds were also docked using the same program. Docking results were visualized using  PyMOL software. LDHA inhibitor candidate is determined by comparing herbal compounds and standard ligand in terms of binding energy, binding site and Lipinski criteria. Result: Oxamate interacting with LDHA had -4.26 ± 0.06 kcal / mol binding energy and bound to six amino acid residues at Gln 99, Arg 105, Asn 137, Arg 168, His 192, and Thr 247. A lower binding energy was observed in 23 herbal compounds and these compounds bound to LDHA at least five amino acid residues like Oxamate. Herbal compounds Phaseolic Acid, Sebacic Acid, D (-) - Fructose, Suberic Acid and Pimelic Acid interacted with amino acid residues of LDHA as same as Oxamate. The other herbal compounds interacted with less or more than six amino acid residues of LDHA. Based on characteristics of five herbal compounds, Phaseolic Acid, Sebacic Acid and Suberic Acid were probably the best candidates of LDHA inhibitor. Conclusion: Phaseolic Acid, Sebacic Acid and Suberic Acid become biocomputationally the best LDHA inhibitor. Enzymatic assays are needed to investigate whether or not all these compounds can inhibit LDHA enzyme activity. Keywords : Cancer, Inhibitor LDHA, Molecular Docking, Herbal Indonesia
Pomegranate Extract Does Not Inhibit Sodium Glucose co-Transporter 2 Protein in Vero Cells Ulfia, Mila; Indarto, Dono; Ayu Sari, Amelya Augusthina; Suselo, Yuliana Heri
Nexus Kedokteran Translasional Vol 6, No 1 (2017): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.209 KB)

Abstract

Backgrounds: Mutation of SLC5A2 gene which encodes sodium glucose co-transporter2 (SGLT2) protein enhances glucose reabsorption on the kidney tubule in some patients with type 2 diabetes (DMT2). Dapagliflozine an oral antidiabetic drug, inhibits SGLT2 activity. Ellagic acid is able to inhibit SGLT2 protein in silico and highly found in pomegranate fruits. The aim of this study was to investigate the effect of pomegranate extracts on glucose levels in a model cell of African green monkey (Vero cell line). Methods: This study was an experimental laboratory with posttest only control group design. 1 x 106 Vero cells perwell were used in five experimental groups: negative control 1 (KKn1), KKn with 20% glucose (KKn2), positive control with dapagliflozine (KKp), ethanol and diethyl ether extract of pomegranate peel (KEDA), methanol extract of pomegranate seeds (BMA). Vero cells were then treated with 125 ppm pomegranate extracts (KEDA and BMA) and incubated for 24 hours. Cell morphology was observed under an inverted microscope with 100 x magnification. Glucose levels in Vero cells were measured using spectrophotometer. Collected data was analyzed descriptively. Result: Morphology of Vero cells was oval, soliter and centered nucleus and did not change during incubation with pomegranate extracts. Glucose levels in Vero cells treated with BMA (28.5 mg/dL) and KEDA (29 mg/dL) were higher than glucose levels in control groups KKp, KKn1, and KKn2 (2.5, 6.5 and 8 mg/dL respectively). Conclusion; Pomegranate extracts do not inhibit SGLT2 protein and increase glucose levels in Vero cells. Purification of pomegranate extracts is required for further investigation of the capability of ellagic acid inhibiting SGLT2 protein. Keywords: Ellagic acid, glucose level, pomegranate, SGLT2, type 2 diabetes.
Screening of Indonesian Pythochemicals as Glucagon-Like Peptide-1 Receptor Agonist In Silico in Type-2 Diabetes Mellitus ., Humamuddin; Wulandari, R Aj Sri; Suselo, Yuliana Heri
Nexus Kedokteran Translasional Vol 6, No 1 (2017): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.209 KB)

Abstract

Background: Prevalence of diabetes mellitus (DM) continues to rise in the world, while DM therapy using oral antidiabetic drug (OAD) was still not effective. GLP-1R agonist was a drug that has been developed because it can lower blood sugar levels without hypoglycemic effect. However, GLP-1R agonists have severe side effects such as pancreatitis, thyroid cancer, and pancreatic cancer. Indonesia has 9,600 herbal plants, some of which have a pharmacological effect that could potentially be developed as a drug. This study aimed to identify the Indonesian pythochemicals that have activity as GLP-1R agonist in silico. Methods: The research was a bioinformatics study which utilized all phytochemicals in HerbalDB that had PubChem access code and met the criteria for Lipinskis rule of five as sample. Truncated Exendin-4 was used as standard compound. The structure of Exendin-4 bound to GLP-1R was obtained from the Protein Data Bank, code: 3C5T. Validation of truncated Exendin-4 with GLP-1R needed to get docking scores and binding site at GLP-1R. Molecular docking between phytochemical compounds with GLP-1R models was done using AutodockVina 1.1.2. Visualization of docking results was done using PyMOL 1.7.4. GLP-1R agonist candidates were analyzed based on binding affinity, binding site similarity, and Lipinskis rule of five criterias. Results: Weighteone and Eupatorin were two selected phytochemicals of the most potentially be GLP-1R agonist based on analysis of docking scores and binding site similarity with truncated Exendin-4. Weighteone and Eupatorin bound to Glu68, Glu127, and Glu128 of GLP-1R. Weighteone had docking scores of -5.12 kcal/mol, -6.22 kcal/mol, and -3.88 kcal/mol lower than truncated Exendin-4. Eupatorin had docking scores of -5.02 kcal/mol, -6 kcal/mol, and -3.58 kcal/mol lower than truncated Exendin-4. Conclusion: Weighteone and Eupatorin were the potential Indonesian phytochemicals that could be a GLP-1R agonist in silico. Future studies using Molecular Dynamics Simulation (MDS) method is required to validate this result. In vitro studies are also needed to evaluate these phytochemicals activity as GLP-1R agonists. Keywords: GLP-1R agonists, Indonesian phytochemicals, molecular docking, diabetes mellitus.
Boeravinone F, Withanolide D, and Chitranone are a Potential Antagonist of Angiotensin Receptor 1 Insilico for Hypertension Treatment Ningtyas, Febri; Wulandari, Sri; ., Balqis
Nexus Kedokteran Translasional Vol 6, No 1 (2017): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.209 KB)

Abstract

Introduction: Hypertension is a silent killer which can cause complications such as heart diseases, stroke, and chronic kidney diseases. Olmesartan is a one of the Angiotensin Receptor Blockers (ARBs) for an alternative treatment of hypertension. However, this drug has low bioavailability, short duration, and partial agonist against angiotensin 1 receptor (AT1). Indonesia has more than 6.000 phytochemicals which are derived from various herbal plants but their therapeutic effects are unknown. Molecular docking is an initial step to find new drug candidates that have shorter time and lower cost. Therefore, this study aimed to identify phytochemicals as an AT1 antagonist through molecular docking for hypertension treatment.  Methods: This was a bioinformatics study. Research samples were all phytochemicals registered in HerbalDB, had 3 dimention structure, and met criteria of Lipinskis rule of five. Olmesartan was used as a standard ligand and obtained from PubChem. AT1 receptor was downloaded from Protein Data Bank. Validation of receptor-standard ligand binding complexes was done by using Autodock Vina 1.1.2 five times. Docking results were visualized using Pymol 1.7 and Chimera 1.10. Data were analyzed using  docking scores, binding sites, molecular conformation, and criteria of Lipinskis rule of five.  Results: Olmesartan had -9.9 Kcal/mol docking score and interacted with the AT1 receptor at Tyr35, Trp84, dan Arg167 residues. Boeravinone F (-10,2 Kcal/mol), Chitranone (-10,5 Kcal/mol), and Withanolide D (-10,8 Kcal/mol) had lower docking score than olmesartan. These phytochemicals had binding sites as same as olmesartan except Chitranone with an additional binding site  at Asp281 residue. The phytochemicals  had different conformation from olmesartan but had similarity of chemical properties with olmesartan.  Conclusions: Boeravinone F, Chitranone, and Withanolide D can be potential as an AT1 receptor inhibitor insilico for hypertension treatment. Keywords: hypertension, AT1 receptor, olmesartan, phytochemicals, molecular docking.
Indonesian Pythochemical as Erythropoietin Agonist In Sillico to Treatment Anemia in Chronic Kidney Disease Kamil, Muhammad Rizki; Suselo, Yuliana Heri; Wulandari, R. Aj Sri
Nexus Kedokteran Translasional Vol 6, No 1 (2017): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.209 KB)

Abstract

Background: Anemia is the most frequent complication in CKD and until now the its treatment still hampered effectiveness and efficiency. Indonesia is known to have 9,600 species of plants that have a pharmacological effect and some compounds have been created for 3D structures and databases. Molecular docking is beginning of the process of the invention the drug most widely used. This study aims to screen Indonesian herbal plant that has activity as an agonist of erythropoietin receptor for treatment of anemia in CKD development with molecular docking method. Methods: The research was a bioinformatics study which utilized all phytochemicals in HerbalDB that had PubChem access code and met the criteria for Lipinskis rule of five as sample. The complex of Epo-EpoR was obtained from the Protein Data Bank, code: 1CN4. Validation of truncated Epo with EpoR needed to get docking scores and binding site at EpoR. Molecular docking between phytochemical compounds with EpoR models was done using AutodockVina 1.1.2. Visualization of docking results was done using PyMOL 1.7.4. Results: There are 12 phytochemicals that have 10 of 17 in common EpoR binding site. There are seven of them met the criteria phytochemical Lipniskis rule of five and then two phytochemicals are selected which has the most variation binding site to EpoR, 18 sites. Gibberellin A51 and Miraxanthin-III were two selected phytochemicals of the most potentially as EpoR agonist based on analysis of docking scores, binding site similarity with truncated Epo, and Lipinskis rule of five criterias. Conclussion: Gibberellin A51 and Miraxanthin-III were the most potent Indonesian phytochemicals that could be a EpoR agonist to development of treatment anemia in CKD. Keywords: Anemia, CKD, EpoR agonists, Indonesian phytochemicals, molecular docking
Screening of Indonesian Pythochemicals as Glucagon-Like Peptide-1 Receptor Agonist In Silico in Type-2 Diabetes Mellitus Humamuddin .; R Aj Sri Wulandari; Yuliana Heri Suselo
Nexus Kedokteran Translasional Vol 6, No 1 (2017): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.209 KB)

Abstract

Background: Prevalence of diabetes mellitus (DM) continues to rise in the world, while DM therapy using oral antidiabetic drug (OAD) was still not effective. GLP-1R agonist was a drug that has been developed because it can lower blood sugar levels without hypoglycemic effect. However, GLP-1R agonists have severe side effects such as pancreatitis, thyroid cancer, and pancreatic cancer. Indonesia has 9,600 herbal plants, some of which have a pharmacological effect that could potentially be developed as a drug. This study aimed to identify the Indonesian pythochemicals that have activity as GLP-1R agonist in silico. Methods: The research was a bioinformatics study which utilized all phytochemicals in HerbalDB that had PubChem access code and met the criteria for Lipinski's rule of five as sample. Truncated Exendin-4 was used as standard compound. The structure of Exendin-4 bound to GLP-1R was obtained from the Protein Data Bank, code: 3C5T. Validation of truncated Exendin-4 with GLP-1R needed to get docking scores and binding site at GLP-1R. Molecular docking between phytochemical compounds with GLP-1R models was done using AutodockVina 1.1.2. Visualization of docking results was done using PyMOL 1.7.4. GLP-1R agonist candidates were analyzed based on binding affinity, binding site similarity, and Lipinski's rule of five criterias. Results: Weighteone and Eupatorin were two selected phytochemicals of the most potentially be GLP-1R agonist based on analysis of docking scores and binding site similarity with truncated Exendin-4. Weighteone and Eupatorin bound to Glu68, Glu127, and Glu128 of GLP-1R. Weighteone had docking scores of -5.12 kcal/mol, -6.22 kcal/mol, and -3.88 kcal/mol lower than truncated Exendin-4. Eupatorin had docking scores of -5.02 kcal/mol, -6 kcal/mol, and -3.58 kcal/mol lower than truncated Exendin-4. Conclusion: Weighteone and Eupatorin were the potential Indonesian phytochemicals that could be a GLP-1R agonist in silico. Future studies using Molecular Dynamics Simulation (MDS) method is required to validate this result. In vitro studies are also needed to evaluate these phytochemicals activity as GLP-1R agonists. Keywords: GLP-1R agonists, Indonesian phytochemicals, molecular docking, diabetes mellitus.
Potency of Indonesian Herbal Compounds as Human Flavin Containing Monooxygenase 3 Inhibitor for Atherosclerosis Prevention Fadhila Balqis Nurfitria; RAJ Sri Wulandari; Dono Indarto
Nexus Kedokteran Translasional Vol 6, No 1 (2017): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.209 KB)

Abstract

Introduction: Atherosclerosis complication in the cardiovascular system has been one of the biggest medical problems in recent years and the definitive treatment has not been found. Trimethylamine-N-oxide (TMAO) is a catalytic product of Flavin-Containing Monooxydase (FMO) 3 enzyme and might act as a predispostion factor for atherosclerosis. Indonesia has many herbal plants which can potentially be developed into antiatherosclerosis drug. This was an initial study of drug development which aimed to identify FMO3 inhibitors from Indonesian herbal plants by using molecular docking. Methods: It was a bioinformatics study which utilized all herbal compounds recorded in HerbalDB, had three dimentional structure, and met the criteria for Lipinski's rule of five. Methimazole was used as a standard ligand and hFMO model was determined using FMO protein template from Methylophaga aminisulfidivorans. Herbal compounds were molecularly docked with hFMO3 models using AutodockVina 1.1.2. PyMOL 1.7 dan Chimera 1.10rc were used for visualization of docking results. Binding affinity, binding site, and Lipinski's rule of five criterias were used to determine hFMO3 inhibitor candidates of herbal compounds. Results: Methimazole bound to the hFMO3 model at Asn194 with binding energy average of -3.8 kcal/mol. Droserone, vanillic acid, (s)-(+) abscisic acid, and sebacic acid had lower binding energy, had similar binding site, and had the best drug like property, compared with methimazole. Conclusion: Droserone, vanillic acid, (s)-(+) absicic acid, and sebacic acid become the potential candidates of hFMO3 inhibitor in silico. A future study using flexible ligand and flexible receptor docking methods is needed to get more accurate results. Keywords: Atherosclerosis, Molecular Docking, FMO3 inhibitor, Indonesian Herbal Plants

Page 1 of 2 | Total Record : 15

 1   2 

Filter by Year

2017 2017


Reset
Filter By Issues
All Issue Vol 6, No 1 (2017): Nexus Kedokteran Translasional Vol 6, No 1 (2017): Nexus Kedokteran Translasional Vol 5, No 2 (2016): Nexus Kedokteran Translasional Vol 5, No 2 (2016): Nexus Kedokteran Translasional Vol 5, No 1 (2016): Nexus Kedokteran Translasional Vol 5, No 1 (2016): Nexus Kedokteran Translasional Vol 4, No 2 (2015): Nexus Kedokteran Translasional Vol 4, No 2 (2015): Nexus Kedokteran Translasional Vol 4, No 1 (2015): Nexus Kedokteran Translasional Vol 4, No 1 (2015): Nexus Kedokteran Translasional Vol 3, No 1 (2014): Nexus Kedokteran Translasional Vol 3, No 1 (2014): Nexus Kedokteran Translasional Vol 2, No 2 (2013): Nexus Kedokteran Translasional Vol 2, No 2 (2013): Nexus Kedokteran Translasional Vol 2, No 1 (2013): Nexus Kedokteran Translasional Vol 2, No 1 (2013): Nexus Kedokteran Translasional Vol 1, No 2 (2012): Nexus Kedokteran Translasional Vol 1, No 2 (2012): Nexus Kedokteran Translasional Vol 1, No 1 (2012): Nexus Kedokteran Translasional Vol 1, No 1 (2012): Nexus Kedokteran Translasional More Issue